The biological cell attachment matrix such as collagen, fibronectin or vitronectin, strongly binds to mammalian cell membrane spanning receptors, and mediates living mammalian cells adhering to the bottom of wells of a polystyrene tissue culture plate, similar to the solid phase of a typical ELISA, in which an antigen is adsorbed to the bottom of wells of a high-bound ELISA plate. Meanwhile, cells growth in the biological cell attachment matrix-coated plates will not alter the cellular morphology and biological characters. Thus, the coated plates allow to detect cell surface and/or cytoplasmic antigens on or in intact living cells (living cell ELISA), cellular function assays, such as cellular metabolism, adhesion, cytotoxicity, migration, proliferation, differentiation, Ion channel flux and report genes, as well as for pharmaceutical drug cytotoxicity, functionality and metabolism assays with a monolayer of intact living cells
| Bio-Coat Plate | Collagen I | Fibronectin | Vitronectin | Laminin | Poly D- or L-Lysine |
| Cell Type | |||||
| HEK-293 | Yes | Yes | Yes | Yes | |
| CHO | Yes | Yes | Yes | ||
| Vero | Yes | Yes | Yes | ||
| BHK | Yes | Yes | Yes | ||
| COS-7 | Yes | ||||
| Primary cells | Yes | Yes | Yes | Yes | |
| HEPG2 | Yes |
| Bio-Coat_Plate | Collagen I | Fibronectin | Vitronectin | Gelatin | Laminin | Collagen I /Fibronectin | Fibronectin /Vitronectin | Fibronectin/ Laminin | Poly D- or L-Lysine |
| Assay Type | |||||||||
| Cell Surface ELISA | Yes | Yes | Yes | Yes | Yes | Yes | |||
| Cell adhesion | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
| Cell cytoxicity | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
| Cell proliferation | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
| Cell differentiation | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | |
| Cell migration | Yes | Yes | Yes | Yes | |||||
| Ion channel/Calcium flux (FLIPR) | Yes | Yes | Yes | ||||||
| Reporter gene | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | |
| Neurite outgrowth | Yes | Yes | Yes | Yes | |||||
| GPCR (Active/Inactive) | Yes | Yes | Yes |
| Catalog_No. | Product | Unit Price |
